Plasmid Preparation:Article Title: Pseudomonas putida KT2440 markerless gene deletion using a combination of λ Red recombineering and Cre/loxP site-specific recombination.
Article Snippet: .. Strain or plasmid Relevant characteristicsa Reference or source Strains P. putida KT2440 Wild type ATCC 12633 E. coli DH10B F− mcrA (mrr-hsdRMS-mcrBC) φ80dlacZ M15 lacX74 deoR recA1 araD139 (ara-leu)7697 galU galK rpsL endA1 nupG Invitrogen E. coli BW25141 F− (araD-araB)567 lacZ4787(del) (::rrnB-3) (phoB-phoR)580 LAM- galU95 uidA3(del)::pir recA1 endA9(del-ins)::FRT rph-1 (rhaD-rhaB)568 rrnB-3 hsdR514 Datsenko and Wanner (2000) Plasmids pBluescript II KS(-) General cloning vector, ApR Invitrogen pKD4 FRT-neo-FRT, R6K, ApR, KanR Datsenko and Wanner (2000) pEX100Tlink Suicide vector, sacB, ApR Quenee, Lamotte and Polack (2005) pJB866 RK2 replicon, xylS–Pm, TcR Blatny et al. (1997) pSCrhaB2 rhaRS–PrhaB, pBBR1 replicon, TMPR Cardona and Valvano (2005) 705-Cre Cre expression plasmid, pSC101, CmR Buchholz, Angrand and Stewart (1996) pBBR1MCS-5 pBBR1 replicon, lacZα, GmR Kovach et al. (1995) pNG247.1 MekR/PmekA, lacZ, pBBR1, AmR Graf and Altenbuchner (2013) pLS1647 pBluescript II KS(-) derivative with loxP-neo-loxP cassette, ApR, KanR This study pLS1648 R6K backbone with loxP-neo-loxP cassette, ApR, KanR This study pNcRed pBluescript II KS(-) derivative with λ Red operon, ApR This study pKsacB pBluescript II KS (-) derivative with sacB, ApR This study pLS2345 pBluescript II KS(-) derivative with λ Red operon and sacB, ApR This study pLS2347 pBluescript II KS(-) derivative with rhaRS–PrhaB, ApR This study pLS2348 pBluescript II KS(-) derivative with cre in which BamHI was mutated, ApR This study pLS2352 pJB866 derivative with λ Red operon and sacB, TcR This study pLS2353 pBluescript II KS(-) derivative with rhaRS–PrhaB–cre, ApR This study pLS2354 pLS2352 derivative with rhaRS–PrhaB–cre, TcR This study pLS2378 pBBR1MCS-5 derivative with rhaRS–PrhaB–cre–sacB, GmR This study pLS3062 pBluescript II KS(-) derivative with mekR–PMekA–cre, ApR This study pLS3063 pLS2352 derivative with mekR–PMekA–cre, TcR This study aAntibiotic resistance phenotypes: AmR, apramycin resistance; ApR, ampicillin resistance; CmR, chloramphenicol resistance; GmR, gentamicin resistance; KanR, kanamycin resistance; TcR, tetracycline resistance; TMPR, trimethoprim resistance. counterselection markers-based gene deletion methods were developed. ..
Laser Capture Microdissection:Article Title: Pseudomonas putida KT2440 markerless gene deletion using a combination of λ Red recombineering and Cre/loxP site-specific recombination.
Article Snippet: .. Strain or plasmid Relevant characteristicsa Reference or source Strains P. putida KT2440 Wild type ATCC 12633 E. coli DH10B F− mcrA (mrr-hsdRMS-mcrBC) φ80dlacZ M15 lacX74 deoR recA1 araD139 (ara-leu)7697 galU galK rpsL endA1 nupG Invitrogen E. coli BW25141 F− (araD-araB)567 lacZ4787(del) (::rrnB-3) (phoB-phoR)580 LAM- galU95 uidA3(del)::pir recA1 endA9(del-ins)::FRT rph-1 (rhaD-rhaB)568 rrnB-3 hsdR514 Datsenko and Wanner (2000) Plasmids pBluescript II KS(-) General cloning vector, ApR Invitrogen pKD4 FRT-neo-FRT, R6K, ApR, KanR Datsenko and Wanner (2000) pEX100Tlink Suicide vector, sacB, ApR Quenee, Lamotte and Polack (2005) pJB866 RK2 replicon, xylS–Pm, TcR Blatny et al. (1997) pSCrhaB2 rhaRS–PrhaB, pBBR1 replicon, TMPR Cardona and Valvano (2005) 705-Cre Cre expression plasmid, pSC101, CmR Buchholz, Angrand and Stewart (1996) pBBR1MCS-5 pBBR1 replicon, lacZα, GmR Kovach et al. (1995) pNG247.1 MekR/PmekA, lacZ, pBBR1, AmR Graf and Altenbuchner (2013) pLS1647 pBluescript II KS(-) derivative with loxP-neo-loxP cassette, ApR, KanR This study pLS1648 R6K backbone with loxP-neo-loxP cassette, ApR, KanR This study pNcRed pBluescript II KS(-) derivative with λ Red operon, ApR This study pKsacB pBluescript II KS (-) derivative with sacB, ApR This study pLS2345 pBluescript II KS(-) derivative with λ Red operon and sacB, ApR This study pLS2347 pBluescript II KS(-) derivative with rhaRS–PrhaB, ApR This study pLS2348 pBluescript II KS(-) derivative with cre in which BamHI was mutated, ApR This study pLS2352 pJB866 derivative with λ Red operon and sacB, TcR This study pLS2353 pBluescript II KS(-) derivative with rhaRS–PrhaB–cre, ApR This study pLS2354 pLS2352 derivative with rhaRS–PrhaB–cre, TcR This study pLS2378 pBBR1MCS-5 derivative with rhaRS–PrhaB–cre–sacB, GmR This study pLS3062 pBluescript II KS(-) derivative with mekR–PMekA–cre, ApR This study pLS3063 pLS2352 derivative with mekR–PMekA–cre, TcR This study aAntibiotic resistance phenotypes: AmR, apramycin resistance; ApR, ampicillin resistance; CmR, chloramphenicol resistance; GmR, gentamicin resistance; KanR, kanamycin resistance; TcR, tetracycline resistance; TMPR, trimethoprim resistance. counterselection markers-based gene deletion methods were developed. ..
Cloning:Article Title: Pseudomonas putida KT2440 markerless gene deletion using a combination of λ Red recombineering and Cre/loxP site-specific recombination.
Article Snippet: .. Strain or plasmid Relevant characteristicsa Reference or source Strains P. putida KT2440 Wild type ATCC 12633 E. coli DH10B F− mcrA (mrr-hsdRMS-mcrBC) φ80dlacZ M15 lacX74 deoR recA1 araD139 (ara-leu)7697 galU galK rpsL endA1 nupG Invitrogen E. coli BW25141 F− (araD-araB)567 lacZ4787(del) (::rrnB-3) (phoB-phoR)580 LAM- galU95 uidA3(del)::pir recA1 endA9(del-ins)::FRT rph-1 (rhaD-rhaB)568 rrnB-3 hsdR514 Datsenko and Wanner (2000) Plasmids pBluescript II KS(-) General cloning vector, ApR Invitrogen pKD4 FRT-neo-FRT, R6K, ApR, KanR Datsenko and Wanner (2000) pEX100Tlink Suicide vector, sacB, ApR Quenee, Lamotte and Polack (2005) pJB866 RK2 replicon, xylS–Pm, TcR Blatny et al. (1997) pSCrhaB2 rhaRS–PrhaB, pBBR1 replicon, TMPR Cardona and Valvano (2005) 705-Cre Cre expression plasmid, pSC101, CmR Buchholz, Angrand and Stewart (1996) pBBR1MCS-5 pBBR1 replicon, lacZα, GmR Kovach et al. (1995) pNG247.1 MekR/PmekA, lacZ, pBBR1, AmR Graf and Altenbuchner (2013) pLS1647 pBluescript II KS(-) derivative with loxP-neo-loxP cassette, ApR, KanR This study pLS1648 R6K backbone with loxP-neo-loxP cassette, ApR, KanR This study pNcRed pBluescript II KS(-) derivative with λ Red operon, ApR This study pKsacB pBluescript II KS (-) derivative with sacB, ApR This study pLS2345 pBluescript II KS(-) derivative with λ Red operon and sacB, ApR This study pLS2347 pBluescript II KS(-) derivative with rhaRS–PrhaB, ApR This study pLS2348 pBluescript II KS(-) derivative with cre in which BamHI was mutated, ApR This study pLS2352 pJB866 derivative with λ Red operon and sacB, TcR This study pLS2353 pBluescript II KS(-) derivative with rhaRS–PrhaB–cre, ApR This study pLS2354 pLS2352 derivative with rhaRS–PrhaB–cre, TcR This study pLS2378 pBBR1MCS-5 derivative with rhaRS–PrhaB–cre–sacB, GmR This study pLS3062 pBluescript II KS(-) derivative with mekR–PMekA–cre, ApR This study pLS3063 pLS2352 derivative with mekR–PMekA–cre, TcR This study aAntibiotic resistance phenotypes: AmR, apramycin resistance; ApR, ampicillin resistance; CmR, chloramphenicol resistance; GmR, gentamicin resistance; KanR, kanamycin resistance; TcR, tetracycline resistance; TMPR, trimethoprim resistance. counterselection markers-based gene deletion methods were developed. ..
Expressing:Article Title: Pseudomonas putida KT2440 markerless gene deletion using a combination of λ Red recombineering and Cre/loxP site-specific recombination.
Article Snippet: .. Strain or plasmid Relevant characteristicsa Reference or source Strains P. putida KT2440 Wild type ATCC 12633 E. coli DH10B F− mcrA (mrr-hsdRMS-mcrBC) φ80dlacZ M15 lacX74 deoR recA1 araD139 (ara-leu)7697 galU galK rpsL endA1 nupG Invitrogen E. coli BW25141 F− (araD-araB)567 lacZ4787(del) (::rrnB-3) (phoB-phoR)580 LAM- galU95 uidA3(del)::pir recA1 endA9(del-ins)::FRT rph-1 (rhaD-rhaB)568 rrnB-3 hsdR514 Datsenko and Wanner (2000) Plasmids pBluescript II KS(-) General cloning vector, ApR Invitrogen pKD4 FRT-neo-FRT, R6K, ApR, KanR Datsenko and Wanner (2000) pEX100Tlink Suicide vector, sacB, ApR Quenee, Lamotte and Polack (2005) pJB866 RK2 replicon, xylS–Pm, TcR Blatny et al. (1997) pSCrhaB2 rhaRS–PrhaB, pBBR1 replicon, TMPR Cardona and Valvano (2005) 705-Cre Cre expression plasmid, pSC101, CmR Buchholz, Angrand and Stewart (1996) pBBR1MCS-5 pBBR1 replicon, lacZα, GmR Kovach et al. (1995) pNG247.1 MekR/PmekA, lacZ, pBBR1, AmR Graf and Altenbuchner (2013) pLS1647 pBluescript II KS(-) derivative with loxP-neo-loxP cassette, ApR, KanR This study pLS1648 R6K backbone with loxP-neo-loxP cassette, ApR, KanR This study pNcRed pBluescript II KS(-) derivative with λ Red operon, ApR This study pKsacB pBluescript II KS (-) derivative with sacB, ApR This study pLS2345 pBluescript II KS(-) derivative with λ Red operon and sacB, ApR This study pLS2347 pBluescript II KS(-) derivative with rhaRS–PrhaB, ApR This study pLS2348 pBluescript II KS(-) derivative with cre in which BamHI was mutated, ApR This study pLS2352 pJB866 derivative with λ Red operon and sacB, TcR This study pLS2353 pBluescript II KS(-) derivative with rhaRS–PrhaB–cre, ApR This study pLS2354 pLS2352 derivative with rhaRS–PrhaB–cre, TcR This study pLS2378 pBBR1MCS-5 derivative with rhaRS–PrhaB–cre–sacB, GmR This study pLS3062 pBluescript II KS(-) derivative with mekR–PMekA–cre, ApR This study pLS3063 pLS2352 derivative with mekR–PMekA–cre, TcR This study aAntibiotic resistance phenotypes: AmR, apramycin resistance; ApR, ampicillin resistance; CmR, chloramphenicol resistance; GmR, gentamicin resistance; KanR, kanamycin resistance; TcR, tetracycline resistance; TMPR, trimethoprim resistance. counterselection markers-based gene deletion methods were developed. ..
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